SrasV1, Main, Exploration, bibRecord, 004469

Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus

Identifieur interne : 004469 ( Main/Exploration ); précédent : 004468; suivant : 004470

Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus

Auteurs : Raymond H. See [Canada] ; Alexander N. Zakhartchouk [Canada] ; Martin Petric [Canada] ; David J. Lawrence [Canada] ; Catherine P. Y. Mok [Canada] ; Robert J. Hogan [États-Unis] ; Thomas Rowe [États-Unis] ; Lois A. Zitzow [États-Unis] ; Karuna P. Karunakaran [Canada] ; Mary M. Hilt [Canada] ; Frank L. Graham [Canada] ; Ludvik Prevec [Canada] ; James B. Mahony [Canada] ; Chetna Sharon [Canada] ; Thierry C. Auperin [Canada] ; James M. Rini [Canada] ; Aubrey J. Tingle [Canada] ; David W. Scheifele [Canada] ; Danuta M. Skowronski [Canada] ; David M. Patrick [Canada] ; Thomas G. Voss [États-Unis] ; Lorne A. Babiuk [Canada] ; Jack Gauldie [Canada] ; Rachel L. Roper [États-Unis] ; Robert C. Brunham [Canada] ; B. Brett Finlay [Canada]

Source :

Journal of general virology [ 0022-1317 ] ; 2006.

RBID : Pascal:06-0150208

Descripteurs français

English descriptors

KwdEn :
- Administration, Intranasal, Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibody Specificity, Disease Models, Animal, Drug Evaluation, Preclinical, Female, Immunoglobulin A (blood), Immunoglobulin A (immunology), Injections, Intramuscular, Injections, Subcutaneous, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Microbiology, Mouse, Neutralization Tests, Nucleocapsid Proteins (genetics), SARS Virus (chemistry), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Vaccination, Vaccine, Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Virology.
MESH :
- chemical , administration & dosage : Vaccines, DNA, Viral Vaccines.
- chemical , blood : Antibodies, Viral, Immunoglobulin A.
- chemical , genetics : Membrane Glycoproteins, Nucleocapsid Proteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Immunoglobulin A, Membrane Glycoproteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Administration, Intranasal, Animals, Antibody Specificity, Disease Models, Animal, Drug Evaluation, Preclinical, Female, Injections, Intramuscular, Injections, Subcutaneous, Mice, Neutralization Tests, Spike Glycoprotein, Coronavirus, Vaccination.

Abstract

Two different severe acute respiratory syndrome (SARS) vaccine strategies were evaluated for their ability to protect against live SARS coronavirus (CoV) challenge in a murine model of infection. A whole killed (inactivated by /?-propiolactone) SARS-CoV vaccine and a combination of two adenovirus-based vectors, one expressing the nucleocapsid (N) and the other expressing the spike (S) protein (collectively designated Ad S/N), were evaluated for the induction of serum neutralizing antibodies and cellular immune responses and their ability to protect against pulmonary SARS-CoV replication. The whole killed virus (WKV) vaccine given subcutaneously to 129S6/SvEv mice was more effective than the Ad S/N vaccine administered either intranasally or intramuscularly in inhibiting SARS-CoV replication in the murine respiratory tract. This protective ability of the WKV vaccine correlated with the induction of high serum neutralizing-antibody titres, but not with cellular immune responses as measured by gamma interferon secretion by mouse splenocytes. Titres of serum neutralizing antibodies induced by the Ad S/N vaccine administered intranasally or intramuscularly were significantly lower than those induced by the WKV vaccine. However, Ad S/N administered intranasally, but not intramuscularly, significantly limited SARS-CoV replication in the lungs. Among the vaccine groups, SARS-CoV-specific IgA was found only in the sera of mice immunized intranasally with Ad S/N, suggesting that mucosal immunity may play a role in protection for the intranasal Ad S/N delivery system. Finally, the sera of vaccinated mice contained antibodies to S, further suggesting a role for this protein in conferring protective immunity against SARS-CoV infection.

Affiliations:

Le document en format XML

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<series><title level="j" type="main">Journal of general virology</title>
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<term>Immunoglobulin A</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Glycoprotéines membranaires</term>
<term>Immunoglobuline A</term>
<term>Protéines de l'enveloppe virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline A</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term>
<term>Animals</term>
<term>Antibody Specificity</term>
<term>Disease Models, Animal</term>
<term>Drug Evaluation, Preclinical</term>
<term>Female</term>
<term>Injections, Intramuscular</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
<term>Neutralization Tests</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Administration par voie nasale</term>
<term>Animaux</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Injections musculaires</term>
<term>Injections sous-cutanées</term>
<term>Modèles animaux de maladie humaine</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Tests de neutralisation</term>
<term>Vaccination</term>
<term>Virus du SRAS</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Vaccin</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Évaluation préclinique de médicament</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Two different severe acute respiratory syndrome (SARS) vaccine strategies were evaluated for their ability to protect against live SARS coronavirus (CoV) challenge in a murine model of infection. A whole killed (inactivated by /?-propiolactone) SARS-CoV vaccine and a combination of two adenovirus-based vectors, one expressing the nucleocapsid (N) and the other expressing the spike (S) protein (collectively designated Ad S/N), were evaluated for the induction of serum neutralizing antibodies and cellular immune responses and their ability to protect against pulmonary SARS-CoV replication. The whole killed virus (WKV) vaccine given subcutaneously to 129S6/SvEv mice was more effective than the Ad S/N vaccine administered either intranasally or intramuscularly in inhibiting SARS-CoV replication in the murine respiratory tract. This protective ability of the WKV vaccine correlated with the induction of high serum neutralizing-antibody titres, but not with cellular immune responses as measured by gamma interferon secretion by mouse splenocytes. Titres of serum neutralizing antibodies induced by the Ad S/N vaccine administered intranasally or intramuscularly were significantly lower than those induced by the WKV vaccine. However, Ad S/N administered intranasally, but not intramuscularly, significantly limited SARS-CoV replication in the lungs. Among the vaccine groups, SARS-CoV-specific IgA was found only in the sera of mice immunized intranasally with Ad S/N, suggesting that mucosal immunity may play a role in protection for the intranasal Ad S/N delivery system. Finally, the sera of vaccinated mice contained antibodies to S, further suggesting a role for this protein in conferring protective immunity against SARS-CoV infection.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>États-Unis</li>
</country>
<region><li>Alabama</li>
<li>Caroline du Nord</li>
<li>Ontario</li>
</region>
<settlement><li>Hamilton (Ontario)</li>
<li>Toronto</li>
</settlement>
<orgName><li>Université McMaster</li>
<li>Université de Toronto</li>
</orgName>
</list>
<tree><country name="Canada"><noRegion><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H." last="See">Raymond H. See</name>
</noRegion>
<name sortKey="Auperin, Thierry C" sort="Auperin, Thierry C" uniqKey="Auperin T" first="Thierry C." last="Auperin">Thierry C. Auperin</name>
<name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A." last="Babiuk">Lorne A. Babiuk</name>
<name sortKey="Brunham, Robert C" sort="Brunham, Robert C" uniqKey="Brunham R" first="Robert C." last="Brunham">Robert C. Brunham</name>
<name sortKey="Finlay, B Brett" sort="Finlay, B Brett" uniqKey="Finlay B" first="B. Brett" last="Finlay">B. Brett Finlay</name>
<name sortKey="Gauldie, Jack" sort="Gauldie, Jack" uniqKey="Gauldie J" first="Jack" last="Gauldie">Jack Gauldie</name>
<name sortKey="Graham, Frank L" sort="Graham, Frank L" uniqKey="Graham F" first="Frank L." last="Graham">Frank L. Graham</name>
<name sortKey="Hilt, Mary M" sort="Hilt, Mary M" uniqKey="Hilt M" first="Mary M." last="Hilt">Mary M. Hilt</name>
<name sortKey="Karunakaran, Karuna P" sort="Karunakaran, Karuna P" uniqKey="Karunakaran K" first="Karuna P." last="Karunakaran">Karuna P. Karunakaran</name>
<name sortKey="Lawrence, David J" sort="Lawrence, David J" uniqKey="Lawrence D" first="David J." last="Lawrence">David J. Lawrence</name>
<name sortKey="Mahony, James B" sort="Mahony, James B" uniqKey="Mahony J" first="James B." last="Mahony">James B. Mahony</name>
<name sortKey="Mok, Catherine P Y" sort="Mok, Catherine P Y" uniqKey="Mok C" first="Catherine P. Y." last="Mok">Catherine P. Y. Mok</name>
<name sortKey="Patrick, David M" sort="Patrick, David M" uniqKey="Patrick D" first="David M." last="Patrick">David M. Patrick</name>
<name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name>
<name sortKey="Prevec, Ludvik" sort="Prevec, Ludvik" uniqKey="Prevec L" first="Ludvik" last="Prevec">Ludvik Prevec</name>
<name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M." last="Rini">James M. Rini</name>
<name sortKey="Scheifele, David W" sort="Scheifele, David W" uniqKey="Scheifele D" first="David W." last="Scheifele">David W. Scheifele</name>
<name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name>
<name sortKey="Skowronski, Danuta M" sort="Skowronski, Danuta M" uniqKey="Skowronski D" first="Danuta M." last="Skowronski">Danuta M. Skowronski</name>
<name sortKey="Tingle, Aubrey J" sort="Tingle, Aubrey J" uniqKey="Tingle A" first="Aubrey J." last="Tingle">Aubrey J. Tingle</name>
<name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N." last="Zakhartchouk">Alexander N. Zakhartchouk</name>
</country>
<country name="États-Unis"><region name="Alabama"><name sortKey="Hogan, Robert J" sort="Hogan, Robert J" uniqKey="Hogan R" first="Robert J." last="Hogan">Robert J. Hogan</name>
</region>
<name sortKey="Roper, Rachel L" sort="Roper, Rachel L" uniqKey="Roper R" first="Rachel L." last="Roper">Rachel L. Roper</name>
<name sortKey="Rowe, Thomas" sort="Rowe, Thomas" uniqKey="Rowe T" first="Thomas" last="Rowe">Thomas Rowe</name>
<name sortKey="Voss, Thomas G" sort="Voss, Thomas G" uniqKey="Voss T" first="Thomas G." last="Voss">Thomas G. Voss</name>
<name sortKey="Zitzow, Lois A" sort="Zitzow, Lois A" uniqKey="Zitzow L" first="Lois A." last="Zitzow">Lois A. Zitzow</name>
</country>
</tree>
</affiliations>
</record>

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Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus

Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus

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